Plasmid

Part:BBa_K4129028:Design

Designed by: Christian Wistrm   Group: iGEM22_DTU-Denmark   (2022-10-08)


Transient expression of fucO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 9306
    Illegal EcoRI site found at 11583
    Illegal XbaI site found at 3066
    Illegal XbaI site found at 3193
    Illegal XbaI site found at 4741
    Illegal XbaI site found at 7485
    Illegal XbaI site found at 8251
    Illegal XbaI site found at 8733
    Illegal XbaI site found at 9501
    Illegal SpeI site found at 7668
    Illegal SpeI site found at 7679
    Illegal SpeI site found at 8043
    Illegal SpeI site found at 8175
    Illegal SpeI site found at 8299
    Illegal SpeI site found at 8406
    Illegal SpeI site found at 8417
    Illegal PstI site found at 141
    Illegal PstI site found at 3997
    Illegal PstI site found at 10450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 9306
    Illegal EcoRI site found at 11583
    Illegal NheI site found at 911
    Illegal NheI site found at 4653
    Illegal SpeI site found at 7668
    Illegal SpeI site found at 7679
    Illegal SpeI site found at 8043
    Illegal SpeI site found at 8175
    Illegal SpeI site found at 8299
    Illegal SpeI site found at 8406
    Illegal SpeI site found at 8417
    Illegal PstI site found at 141
    Illegal PstI site found at 3997
    Illegal PstI site found at 10450
    Illegal NotI site found at 4644
    Illegal NotI site found at 4698
    Illegal NotI site found at 7524
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 9306
    Illegal EcoRI site found at 11583
    Illegal BglII site found at 145
    Illegal BglII site found at 13310
    Illegal BamHI site found at 2347
    Illegal BamHI site found at 3199
    Illegal BamHI site found at 3735
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3595
    Illegal XhoI site found at 9847
    Illegal XhoI site found at 10564
    Illegal XhoI site found at 11042
    Illegal XhoI site found at 14297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 9306
    Illegal EcoRI site found at 11583
    Illegal XbaI site found at 3066
    Illegal XbaI site found at 3193
    Illegal XbaI site found at 4741
    Illegal XbaI site found at 7485
    Illegal XbaI site found at 8251
    Illegal XbaI site found at 8733
    Illegal XbaI site found at 9501
    Illegal SpeI site found at 7668
    Illegal SpeI site found at 7679
    Illegal SpeI site found at 8043
    Illegal SpeI site found at 8175
    Illegal SpeI site found at 8299
    Illegal SpeI site found at 8406
    Illegal SpeI site found at 8417
    Illegal PstI site found at 141
    Illegal PstI site found at 3997
    Illegal PstI site found at 10450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 9306
    Illegal EcoRI site found at 11583
    Illegal XbaI site found at 3066
    Illegal XbaI site found at 3193
    Illegal XbaI site found at 4741
    Illegal XbaI site found at 7485
    Illegal XbaI site found at 8251
    Illegal XbaI site found at 8733
    Illegal XbaI site found at 9501
    Illegal SpeI site found at 7668
    Illegal SpeI site found at 7679
    Illegal SpeI site found at 8043
    Illegal SpeI site found at 8175
    Illegal SpeI site found at 8299
    Illegal SpeI site found at 8406
    Illegal SpeI site found at 8417
    Illegal PstI site found at 141
    Illegal PstI site found at 3997
    Illegal PstI site found at 10450
    Illegal NgoMIV site found at 2983
    Illegal NgoMIV site found at 10584
    Illegal NgoMIV site found at 13775
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3788
    Illegal BsaI site found at 13177
    Illegal BsaI site found at 14123
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 2697
    Illegal BsaI.rc site found at 7712
    Illegal SapI site found at 323


Design Notes

Source

BBa_K4129026 + BBa_K4129008

References